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Researchers Re-Engineer E-Coli DNA to Make it Programmable

A team of synthetic biologists led by Farren Isaacs at Yale University [has taken] Escherichia coli cells and replaced all of the UAG stop codons with UAAs. They also deleted the instructions for making the release factor that usually binds to UAG, effectively rendering UAG meaningless…

The next step was to assign a new meaning to UAG during protein production. The team did this by designing molecules called transfer RNAs and accompanying enzymes that would attach an unnatural amino acid – fed to the cell – wherever they spotted the UAG codon…

By reintroducing UAGs at specific locations, as the Yale team have done, unnatural amino acids can be added into proteins at will.

"We now have an organism that has a new code, and we can reliably and efficiently open up the chemical diversity of proteins," says Isaacs.

For example, artificial amino acids could be added that give proteins unusual properties, such as the ability to bind to metals – resulting in novel adhesives. Or enzymes could be developed that are activated only in the presence of other molecules – which could be useful for drugs. “The genetic code is conserved for all of life, so this is a fundamental step forward,” says Philipp Holliger of the MRC Laboratory of Molecular Biology in Cambridge, UK. He says that because so much of the genetic code is redundant, there might be other codons that could be reassigned to expand the chemistry of living organisms.

(via Reprogrammed bacterium speaks new language of life - life - 23 October 2013 - New Scientist)

Currently, Bajaj’s Boxer brand “commands a market share of 90% in Uganda, 40% in Nigeria, 35% in Angola and 30% in Kenya”, the statement said adding that “one of the main success factors, apart from the quality of the motorbike has been the company’s ability to cater to the service and spare parts requirements of its customers in these countries”.

Bajaj Auto also announced it has teamed up with SOMOCO, a locally-owned Indian company in Ghana and a subsidiary of the Mohinani conglomerate, to distribute its products in its 51st global market.

"Ghana’s motor-bike market is estimated to be about 100,000 units per annum," Bajaj Auto said adding that "demand has grown rapidly in the last few years thanks to a growing middle class. The company aims to be the market leader in three to five years time".

Bajaj Auto said the company would initially target the utility and commuter segment through its immensely successful motorbike brand which comes in two variants - the 100cc version and the powerful 150cc version.

Ugandans and Batswana take note !! Please complete this short survey and help me to help YOU ! http://www.surveymonkey.com/s/5VVYM2X

Northwestern University unveils some shiny, new design proposals for the former Prentice Women’s Hospital site on its Chicago campus.

Designs submitted by Goettsch Partners and Ballinger, Perkins & Will and Adrian Smith & Gordon Gill Architecture and Payette made the final three and now the university wants feedback.

So, tell us: Which design do you like best?

As technical traders, it our job to follow the money flow. Over millions of years there have been cycles, and patterns that have repeated over and ove…

As technical traders, it our job to follow the money flow. Over millions of years there have been cycles, and patterns that have repeated over and over. Take the seasons for example, there is summer, spring, winter, and fall. These seasons tell us when to prepare for cold weather, warm weather, when to plant, when to harvest, and more. The ancient hunters used to watch the footprints of animal tracks to find their lairs, this is how they were able to feed their families. This is basically the same thing that technical traders do with charts.   As a technical trader we look for patterns that repeat over and over again in order to find solid trading opportunities. Often, when that chart pattern appears it gives the technical trader a chance to make a solid investment decision. While technical trading is not perfect it will allow the discipline trader to exit the investment with a small loss when wrong. Yesterday, the Federal Reserve Bank announced that they would not start to taper their current $85 billion a month QE-3 program. Now, many traders were nervous about taking action ahead of the Fed announcement. A few days earlier I alerted the InTheMoneyStocks members to buy the gold mining stocks. This decision had nothing to do with the Federal Reserve or anyone else, it had to do with the charts. We were able to pick up the Market Vectors Gold Miners ETF (NYSEARCA:GDX) at $25.60 a share on September 12, 2013. Many members where asking me if a taper of QE-3 would hurt the gold mining stocks. I said to them that the chart is telling us that the GDX is going to rise and if I’m wrong we will just stop out of the position with a small loss. I said, trust the charts and do not worry about the news and the chatter in the media. Fortunately for us the GDX rallied higher by 10 percent yesterday closing above $28.00 a share. Learn to use the charts. Over the years the charts have usually forecasted most market moves before anyone in the media. Technical traders should also have a stop loss in place just in case the pattern on the chart fails, but the loss should be small. The key to trading is to let the winning trades run to target and cut the losing quickly when the chart pattern tells us to do so. Now there is a lot to learn about the charts, you will not master them in a day. In fact, we are always learning when following charts, but it sure beats trying to trade off of the news or the talking heads in the media. Nicholas Santiago InTheMoneyStocks.com

Blue sunset by MiljanMladenovic

Last night, JP Morgan was about to roll out a Q&A with #AskJPM and things went wrong.. very wrong.

More highlights here: trib.in/1bqIdEN

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Chipotle Mexican Grill, Inc. (NYSE:CMG) has recently run into an epic trend line that signals a longer term top in the stock. This trend line stretche…

Chipotle Mexican Grill, Inc. (NYSE:CMG) has recently run into an epic trend line that signals a longer term top in the stock. This trend line stretches back to 2007, 2012 and now 2013. Each time this level has been reached, the stock has pull back significantly. A pull back over the next 6 months could yield an easy target of $450.00. Gareth Soloway InTheMoneyStocks.com